Biomedical Electron Microscopy

Biomedical Electron Microscopy

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This comprehensive reference illustrates optimal preparation methods in biological electron microscopy compared with common methodological problems. Not only will the basic methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be presented, but the authors also endeavor to illustrate more specialized techniques such as negative staining, autoradiography, cytochemistry, immunoelectron microscopy, and computer-assisted image analysis. Authored by the key leaders in the biological electron microscopy field Illustrates both optimal and suboptimal or artifactual results in a variety of electron microscopy disciplines Introduces students on how to read and interpret electron micrographsSDS-digested freeze-fracture replica labeling electron microscopy to study the two-dimensional distribution of integral membrane proteins and ... In a€œMethods in Plant Cell Biologya€ (D. W. Galbraith, H. J. Bohnert, and D. P. Bourque, eds.) ... Protocol for 3-D visualization of molecules on mica via the quick-freeze, deep- etch technique. ... The interpretation of pictures of freeze-fractured biological material.

Title:Biomedical Electron Microscopy
Author: Arvid B. Maunsbach, Björn A. Afzelius
Publisher:Academic Press - 1998-11-03

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